The web server can be used for small-scale data sets (at most 100 substitutions, with no input sequence of length > 35,000 residues). The webserver requires each FASTA format input, wherein each mutation is represented by wildtype and mutant protein sequences, as well as a valid email address.
The standalone executable can be used for genome-scale data sets. As input, the MutPredLOF software requires the output file from ANNOVAR's coding_change.pl program. To install and run MutPredLOF, you will need about 16 GB of hard disk space and about 8 GB RAM. Click on the link below to download.
Before downloading: please read the MutPredLOF license agreement.